What is ribosomal multilocus sequence typing?

Ribosomal Multilocus Sequence Typing (rMLST) is an approach that indexes variation of the 53 genes encoding the bacterial ribosome protein subunits (rps genes) as a means of integrating microbial taxonomy and typing.

What is multilocus sequence typing used for?

Multilocus sequence typing (MLST) is an unambiguous procedure for characterising isolates of bacterial species using the sequences of internal fragments of (usually) seven house-keeping genes.

How are plasmids sequenced?

Methods for plasmid detection There are several approaches for plasmid detection, based on PCR, gel electrophoresis, optical mapping or sequencing. PCR-based replicon typing. This approach targets the conserved replicon sites of plasmid, and multiplex PCR can be expanded to simultaneously target many replicons.

What is whole genome multilocus sequence typing?

Whole genome multilocus sequence typing (wgMLST) is a recently developed methodology that simplifies genomic analyses by transforming millions of base pairs of sequence into character data for each gene. We sequenced 13 US Y. pestis isolates with known epidemiologic relationships.

What is Multilocus sequence analysis?

Multilocus sequence analysis (MLSA) is a phylogenetic analysis of multiple internal fragments of genes that are ubiquitous to the studied taxon, present as a single copy within the genome, and are not subject to selective pressure (27).

What is MLST analysis?

Multilocus sequence typing (MLST) is a molecular typing technique whereby a number of well chosen housekeeping genes (loci) are sequenced, usually in part. In a typical MLST approach, recombination is expected to occur with a much higher frequency than point mutations.

What is core genome Multilocus sequence?

Core genome multilocus sequence typing (cgMLST) aims to combine the discriminatory power of classical multilocus sequence typing (MLST) with the extensive genetic data obtained by WGS. By sequencing a representative collection of 146 E.

What is the difference between MLST and MLSA?

Moreover, MLST is usually applied to strains that belong to a well-defined species while MLSA is more often used when species boundaries are not well known and MLSA data are used to improve species descriptions.

What is a sequence type in genetics?

Sequencing DNA means determining the order of the four chemical building blocks – called “bases” – that make up the DNA molecule. The sequence tells scientists the kind of genetic information that is carried in a particular DNA segment.

What is rMLST?

We propose Ribosomal Multi Locus Sequence Typing (rMLST), an approach which indexes variation of the 53 genes encoding the bacterial ribosome protein subunits (rps genes), as a means of integrated microbial taxonomy and typing.

How far can a primer sequence?

18 and 24 bases
Good sequencing results require high quality primers, just as much as high quality templates. The following criteria are considered most critical in sequencing primer design: Primer length should be in the range of 18 and 24 bases.

What is Multilocus sequence analysis MLSA?

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