What is antibiotic resistance gene pUC19?
ampicillin resistance gene
In addition to β-galactosidase, pUC19 also encodes for an ampicillin resistance gene (ampR), via a β-lactamase enzyme that functions by degrading ampicillin and reducing its toxicity to the host. The ori site, or origin of replication, is derived from the plasmid pMB1. pUC19 is small but has a high copy number.
Which antibiotic resistance marker is present in pUC19 vector?
ampicillin resistance
(A) The bla gene in pUC19, which confers ampicillin resistance, was replaced with fabI and its promoter region (pFab).
What is difference between pUC18 and pUC19 vector?
pUC19 is identical to pUC18 except that they contain multiple cloning sites (MCS) arranged in opposite orientations. pUC18/19 plasmids contain: 1. The pMB1 replicon rep responsible for the replication of plasmid (source – plasmid pBR322).
Which selection system is used in the pUC19 plasmid?
9. Which selection system is used in the Puc8 plasmid? Explanation: The identification of recombinants produced by Puc8 plasmid is done by insertional inactivation of lacz’ gene, distinction is done on the basis of the observed colour of the colonies (blue or white).
Which selection system is used in the pUC19 plasmid Mcq?
What is pUC19 plasmid used for?
pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number.
What is pUC19 vector used for?
pUC19 Vector. pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1).
What is the origin of replication in pUC19?
The ori site, or origin of replication, is derived from the plasmid pMB1. pUC19 is small but has a high copy number. The high copy number is a result of the lack of the rop gene and a single point mutation in the ori of pMB1. The lacZ gene codes for β-galactosidase.
What is the mechanism of antibiotic resistance?
The production of enzymes capable of introducing chemical changes to the antimicrobial molecule is a well-known mechanism of acquired antibiotic resistance in both gram-negative and gram-positive bacteria.
What is the mechanism of penicillin resistance to Staphylococcus aureus?
Infections caused by penicillin-resistant S. aureusbecame clinically relevant after penicillin became widely available and the mechanism of resistance was found to be a plasmid-encoded penicillinase that was readily transmitted between S. aureusstrains, resulting in rapid dissemination of the resistance trait (17).