What is the function of let-7?
The let-7 microRNAs regulate the cell proliferation, cell cycle, apoptosis, metabolism, and stemness. In CRC, expressions of let-7 microRNAs have been reported to be reduced, and so let-7 microRNAs are considered to be a tumor suppressor.
How does LIN28 let-7 control development and disease?
During embryonic development Lin28 levels decline allowing an accumulation of let-7 miRNAs. let-7 induction inhibits the self-renewal of undifferentiated cells and promotes differentiation. Introduction of synthetic let-7 is sufficient to rescue the compromised differentiation phenotype of miRNA-deficient ESCs[20].
What does LIN28 stand for?
undifferentiated human embryonic stem cells
LIN28 is a marker of undifferentiated human embryonic stem cells and has been used to enhance the efficiency of the formation of iPS cells from human fibroblasts.
What is the role of the LET-7 family of miRNA in human embryonic stem cell pluripotency?
The let-7 family of microRNAs (miRNAs), first identified in C. elegans but functionally conserved from worms to human, constitutes an important class of regulators for diverse cellular functions ranging from cell proliferation, differentiation and pluripotency to cancer development and progression.
What is the role of the LET 7 family of Mirna in human embryonic stem cell pluripotency?
Is LIN28A real?
LIN28A (Lin-28 Homolog A) is a Protein Coding gene. Diseases associated with LIN28A include Embryonal Tumor With Multilayered Rosettes and Atypical Teratoid Rhabdoid Tumor. Among its related pathways are Nervous system development and CCL18 signaling pathway.
What is SOX2 used for?
The SOX2 gene provides instructions for making a protein that plays a critical role in the formation of many different tissues and organs during embryonic development. The SOX2 protein is especially important for the development of the eyes.
What is SOX2 marker for?
SOX2 is a marker for NCSs both during development and in the adult brain (Ellis et al., 2004; Suh et al., 2007) and is essential for the viability of NSCs both in vivo and in vitro (Favaro et al., 2009) (our unpublished observations).
How many microRNAs are in the human genome?
Human genome encodes approximately 2,600 mature microRNAs (miRBase v. 22) and, according to GENCODE data (v. 29), more than 200,000 of transcripts, including isoforms with slight variations.
What activates SOX2?
Sox2 protein can be modulated by methylation, acetylation, sumoylation and phosphorylation, which subsequently affect its activities as a transcriptional regulator.
How many types of miRNA are there?
There are now over 2000 miRNAs that have been discovered in humans and it is believed that they collectively regulate one third of the genes in the genome. miRNAs have been linked to many human diseases and are being pursued as clinical diagnostics and as therapeutic targets.
How is SOX2 regulated?
Expression and function of SOX2 is regulated through several post-translational modifications such as ubiquitination, sumoylation, phosphorylation, methylation and acetylation.
What does HMGA2 overexpression of let-7 mean?
“Overexpression of HMGA2 relates to reduction of the let-7 and its relationship to clinicopathological features in pituitary adenomas”. Mod Pathol. 22 (3): 431–41. doi: 10.1038/modpathol.2008.202. PMID 19136928.
Is let-7 miRNA consensus binding site 1 critical for HMGA2 transcriptional upregulation?
“Identification of novel HMGA2 fusion sequences in lipoma: evidence that deletion of let-7 miRNA consensus binding site 1 in the HMGA2 3′ UTR is not critical for HMGA2 transcriptional upregulation”. Genes Chromosomes Cancer. 48 (8): 673–8. doi: 10.1002/gcc.20674. PMID 19431195. S2CID 5328884.
Does let-7 regulate the lin-41 homologue of Drosophila?
“Regulation of the Drosophila lin-41 homologue dappled by let-7 reveals conservation of a regulatory mechanism within the LIN-41 subclade”. Dev Dyn. 237 (1): 196–208. doi: 10.1002/dvdy.21396.
How is the time of appearance of let-7 microRNA transcriptionally controlled?
“The time of appearance of the C. elegans let-7 microRNA is transcriptionally controlled utilizing a temporal regulatory element in its promoter”. Dev Biol. 259 (2): 364–79. doi: 10.1016/S0012-1606 (03)00202-1. PMID 12871707. Pasquinelli AE, McCoy A, Jiménez E, Saló E, Ruvkun G, Martindale MQ, Baguñà J (2003).